human kidney ffpe tissue sections (Cybrdi Inc)
Structured Review

Human Kidney Ffpe Tissue Sections, supplied by Cybrdi Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+kidney+ffpe+tissue+sections/human+kidney+ffpe+tissue+sections/pmc03821180-141-1-6
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Comprehensive comparative analysis of RNA sequencing methods for degraded or low input samples"
Article Title: Comprehensive comparative analysis of RNA sequencing methods for degraded or low input samples
Journal: Nature methods
doi: 10.1038/nmeth.2483
Figure Legend Snippet: Shown are key metrics for RNase-H (orange), Ribo-Zero (pink) and total (black) libraries from pancreas and FFPE kidney RNA. ( a ) Percent of rRNA mapping reads; ( b ) Proportion of reads mapping to exons (solid), introns (hatched), and intergenic (white) regions; ( c ) mean coefficient of variation ( y axis) for the top 1,000 expressed transcripts in each library ( x axis); ( d ) Pearson correlation coefficient between each library and a control Total library.
Techniques Used: Control
Related Articles
Formalin-fixed Paraffin-Embedded:Article Title: Comprehensive comparative analysis of RNA sequencing methods for degraded or low input samples Article Snippet: To prepare fragmented RNA from high quality human K-562 RNA (RNA Integrity Number (RIN) 9.0; Ambion), we mixed 10 μg of K-562 total RNA (Ambion) with 2 μl of ERCC RNA Spike-In Control Mix 1 (1:10, Ambion), heated in 1× fragmentation buffer (Affymetrix) at 85°C for 3 minutes, quickly chilled on ice, and purified with 2.2× RNAClean SPRI beads. .. For Isolation:Article Title: Comprehensive comparative analysis of RNA sequencing methods for degraded or low input samples Article Snippet: To prepare fragmented RNA from high quality human K-562 RNA (RNA Integrity Number (RIN) 9.0; Ambion), we mixed 10 μg of K-562 total RNA (Ambion) with 2 μl of ERCC RNA Spike-In Control Mix 1 (1:10, Ambion), heated in 1× fragmentation buffer (Affymetrix) at 85°C for 3 minutes, quickly chilled on ice, and purified with 2.2× RNAClean SPRI beads. .. For Purification:Article Title: Comprehensive comparative analysis of RNA sequencing methods for degraded or low input samples Article Snippet: To prepare fragmented RNA from high quality human K-562 RNA (RNA Integrity Number (RIN) 9.0; Ambion), we mixed 10 μg of K-562 total RNA (Ambion) with 2 μl of ERCC RNA Spike-In Control Mix 1 (1:10, Ambion), heated in 1× fragmentation buffer (Affymetrix) at 85°C for 3 minutes, quickly chilled on ice, and purified with 2.2× RNAClean SPRI beads. .. For |